Anti-EZH2 Polyclonal Antibody (HS927014)

Name: Anti-EZH2 Polyclonal Antibody
Brand: abinScience
SKU: HS927014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HS927014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human EZH2 (Arg608-Pro746).

Cible

ENX-1, Enhancer of zeste homolog 2, EZH2, Lysine N-methyltransferase 6, KMT6, Histone-lysine N-methyltransferase EZH2

Purification

Purified by antigen affinity column.

Numéro d'accès

Q15910

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Histone-lysine N-methyltransferase EZH2 is a ~85 kDa protein. Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems.

1. Bracken, AP. et al. (2003) The EMBO journal 22, 5323-35. PMID: 14532106

2. Cao, R. et al. (2004) Molecular cell 15, 57-67. PMID: 15225548

3. Pasini, D. et al. (2004) The EMBO journal 23, 4061-71. PMID: 15385962

4. Bracken, AP. et al. (2006) Genes & development 20, 1123-36. PMID: 16618801

5. Kim, DH. et al. (2006) Nature structural & molecular biology 13, 793-7. PMID: 16936726

6. Bracken, AP. et al. (2007) Genes & development 21, 525-30. PMID: 17344414

7. McCabe, MT. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2989-94. PMID: 22323599

8. Chu, CS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 1355-60. PMID: 24474760

9. Kim, JM. et al. (2015) Scientific reports 5, 16714. PMID: 26581166

10. Li, D. et al. (2018) Nature communications 9, 2829. PMID: 30026490

Note

For research use only.

Images

Reférences

Fiche technique

Anti-EZH2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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