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Anti-Human Coagulation Factor II Polyclonal Antibody (HF621014)

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Anti-Human Coagulation Factor II Polyclonal Antibody
Anti-Human Coagulation Factor II Polyclonal Antibody
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Vista general
Número de catálogoHF621014
Descripción
Anti-F2 Polyclonal Antibody (HF621014) is a rabbit polyclonal antibody detecting F2 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human F2 (Ala44-Arg327).
Objetivo F2, Coagulation factor II, Prothrombin
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso P00734
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Prothrombin (F2/PIVKA-II) is a ~70 kDa protein. Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells.

1. Naito, K. et al. (1991) The Journal of biological chemistry 266, 7353-8. PMID: 2019570
2. Choi, SH. et al. (2011) Blood 118, 6963-70. PMID: 21976677
3. Shinozawa, E. et al. (2018) Frontiers in pharmacology 9, 1431. PMID: 30568593
4. Senden, NH. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 4318-24. PMID: 9780208
Nota For research use only.
Imágenes
  • Anti-Human Coagulation Factor II Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-F2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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