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Anti-FADS3 Polyclonal Antibody (HV205014)

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Vista general
Número de catálogoHV205014
Descripción
Anti-FADS3 Polyclonal Antibody (HV205014) is a rabbit polyclonal antibody detecting FADS3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human FADS3 (Glu331-Gln445).
Objetivo Delta(13) fatty acid desaturase, Delta(13) desaturase, FADS3, Fatty acid desaturase 3, CYB5RP
Purificación Purified by antigen affinity column.
Número de acceso Q9Y5Q0
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Fatty acid desaturase 3 (FADS3) is a ~51 kDa protein. Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base that comprises mammalian ceramides is sphing-4-enine (sphingosine or SPH) which has a trans (E) desaturation at carbon 4. FADS3 is a desaturase that introduces a cis (Z) double bond between carbon 14 and carbon 15 of the sphingoid base (also known as long chain base, LCB), producing LCBs such as sphinga-4,14-dienine (SPD, d18:2(4E,14Z)) from SPH. Prefers SPH-containing ceramides (N-acylsphing-4-enines) as substrates. Capable of metabolizing also the SPH in its free form.

1. Karsai, G. et al. (2020) The Journal of biological chemistry 295, 1889-1897. PMID: 31862735
2. Jojima, K. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 3318-3335. PMID: 31916624
3. Jojima, K. et al. (2023) Biochimica et biophysica acta. Molecular and cell biology of lipids 1868, 159335. PMID: 37209771
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-FADS3 Polyclonal Antibody

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