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Anti-FAHD1 Polyclonal Antibody (HB329014)

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Anti-FAHD1 Polyclonal Antibody
Anti-FAHD1 Polyclonal Antibody
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Vista general
Número de catálogoHB329014
Descripción
Anti-FAHD1 Polyclonal Antibody (HB329014) is a rabbit polyclonal antibody detecting mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human FAHD1 (Ala25-Tyr221).
Objetivo Oxaloacetate decarboxylase, mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q6P587, Q2HJ98, Q6AYQ8, Q8R0F8
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Oxaloacetate tautomerase FAHD1, mitochondrial is a ~24 kDa protein. Tautomerase that converts enol-oxaloacetate, a strong inhibitor of succinate dehydrogenase, to the physiological keto form of oxaloacetate. It is thereby required to maximize aerobic respiration efficiency by preventing succinate dehydrogenase inhibition. Also acts as a weak oxaloacetate decarboxylase (ODx), catalyzing the decarboxylation of oxaloacetate (OAA) to pyruvate and CO(2), and as such is likely a regulatory enzyme in the TCA cycle. Also displays acylpyruvase activity, being able to hydrolyze acetylpyruvate and fumarylpyruvate in vitro. Exhibits only a weak hydrolase activity on methylacetopyruvate and acetylacetone, and no activity toward acetoacetyl-CoA.

1. Zmuda, AJ. et al. (2024) Nature communications 15, 846. PMID: 38287013
2. Pircher, H. et al. (2015) The Journal of biological chemistry 290, 6755-62. PMID: 25575590
3. Weiss, AKH. et al. (2018) The Biochemical journal 475, 3561-3576. PMID: 30348641
4. Pircher, H. et al. (2011) The Journal of biological chemistry 286, 36500-8. PMID: 21878618
Nota For research use only
Imágenes
  • Anti-FAHD1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FAHD1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: 293T cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Mouse kidney lysate
    Lane 4: Rat kidney lysate
    Lane 5: Mouse liver lysate
    Lane 6: Rat liver lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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