Anti-FAHD1 Polyclonal Antibody (HB329014)

Name: Anti-FAHD1 Polyclonal Antibody
Brand: abinScience
SKU: HB329014
Price: 96 USD
Availability: InStock

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Fiche technique
SDS

Aperçu

Numéro de catalogue

HB329014

Description

Anti-FAHD1 Polyclonal Antibody (HB329014) is a rabbit polyclonal antibody detecting mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human FAHD1 (Ala25-Tyr221).

Cible

Oxaloacetate decarboxylase, mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q6P587, Q2HJ98, Q6AYQ8, Q8R0F8

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Oxaloacetate tautomerase FAHD1, mitochondrial is a ~24 kDa protein. Tautomerase that converts enol-oxaloacetate, a strong inhibitor of succinate dehydrogenase, to the physiological keto form of oxaloacetate. It is thereby required to maximize aerobic respiration efficiency by preventing succinate dehydrogenase inhibition. Also acts as a weak oxaloacetate decarboxylase (ODx), catalyzing the decarboxylation of oxaloacetate (OAA) to pyruvate and CO(2), and as such is likely a regulatory enzyme in the TCA cycle. Also displays acylpyruvase activity, being able to hydrolyze acetylpyruvate and fumarylpyruvate in vitro. Exhibits only a weak hydrolase activity on methylacetopyruvate and acetylacetone, and no activity toward acetoacetyl-CoA.

1. Zmuda, AJ. et al. (2024) Nature communications 15, 846. PMID: 38287013

2. Pircher, H. et al. (2015) The Journal of biological chemistry 290, 6755-62. PMID: 25575590

3. Weiss, AKH. et al. (2018) The Biochemical journal 475, 3561-3576. PMID: 30348641

4. Pircher, H. et al. (2011) The Journal of biological chemistry 286, 36500-8. PMID: 21878618

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-FAHD1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Mouse kidney lysate
Lane 4: Rat kidney lysate
Lane 5: Mouse liver lysate
Lane 6: Rat liver lysate

Reférences

Fiche technique

Anti-FAHD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-FAHD1 Polyclonal Antibody (HB329014)

Aperçu

Images

Reférences

Fiche technique

SDS