Anti-FAHD1 Polyclonal Antibody (HB329014)

Name: Anti-FAHD1 Polyclonal Antibody
Brand: abinScience
SKU: HB329014
Price: 96 USD
Availability: InStock

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Каталожный номер

HB329014

Описание

Anti-FAHD1 Polyclonal Antibody (HB329014) is a rabbit polyclonal antibody detecting mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant human FAHD1 (Ala25-Tyr221).

Цель

Oxaloacetate decarboxylase, mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

Q6P587, Q2HJ98, Q6AYQ8, Q8R0F8

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Oxaloacetate tautomerase FAHD1, mitochondrial is a ~24 kDa protein. Tautomerase that converts enol-oxaloacetate, a strong inhibitor of succinate dehydrogenase, to the physiological keto form of oxaloacetate. It is thereby required to maximize aerobic respiration efficiency by preventing succinate dehydrogenase inhibition. Also acts as a weak oxaloacetate decarboxylase (ODx), catalyzing the decarboxylation of oxaloacetate (OAA) to pyruvate and CO(2), and as such is likely a regulatory enzyme in the TCA cycle. Also displays acylpyruvase activity, being able to hydrolyze acetylpyruvate and fumarylpyruvate in vitro. Exhibits only a weak hydrolase activity on methylacetopyruvate and acetylacetone, and no activity toward acetoacetyl-CoA.

1. Zmuda, AJ. et al. (2024) Nature communications 15, 846. PMID: 38287013

2. Pircher, H. et al. (2015) The Journal of biological chemistry 290, 6755-62. PMID: 25575590

3. Weiss, AKH. et al. (2018) The Biochemical journal 475, 3561-3576. PMID: 30348641

4. Pircher, H. et al. (2011) The Journal of biological chemistry 286, 36500-8. PMID: 21878618

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-FAHD1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Mouse kidney lysate
Lane 4: Rat kidney lysate
Lane 5: Mouse liver lysate
Lane 6: Rat liver lysate

Ссылки

Даташит

Anti-FAHD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-FAHD1 Polyclonal Antibody (HB329014)

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