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Anti-FHIT Polyclonal Antibody (HB415014)

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Aperçu
Numéro de catalogueHB415014
Description
Anti-FHIT Polyclonal Antibody (HB415014) is a rabbit polyclonal antibody detecting P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human FHIT (Met1-Gln147).
Cible Bis(5'-adenosyl)-triphosphatase; 3.6.1.29; AP3A hydrolase; AP3Aase; Adenosine 5'-monophosphoramidase FHIT; 3.9.1.-; Adenylylsulfatase; 3.6.2.1; Adenylylsulfate-ammonia adenylyltransferase; 2.7.7.51; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P49789, O89106, Q1KZG4, Q9JIX3
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Bis(5'-adenosyl)-triphosphatase (FHIT) is a ~16 kDa protein. Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2.

1. Trapasso, F. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1592-7. PMID: 12574506
2. Huang, K. et al. (2004) Biochemistry 43, 7637-42. PMID: 15182206
3. Barnes, LD. et al. (1996) Biochemistry 35, 11529-35. PMID: 8794732
4. Lima, CD. et al. (1997) Science (New York, N.Y.) 278, 286-90. PMID: 9323207
5. Brenner, C. et al. (1997) Protein engineering 10, 1461-3. PMID: 9543008
6. Pace, HC. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5484-9. PMID: 9576908
9. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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