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Обзор
Каталожный номерHB415014
Описание
Anti-FHIT Polyclonal Antibody (HB415014) is a rabbit polyclonal antibody detecting P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant human FHIT (Met1-Gln147).
Цель Bis(5'-adenosyl)-triphosphatase; 3.6.1.29; AP3A hydrolase; AP3Aase; Adenosine 5'-monophosphoramidase FHIT; 3.9.1.-; Adenylylsulfatase; 3.6.2.1; Adenylylsulfate-ammonia adenylyltransferase; 2.7.7.51; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P49789, O89106, Q1KZG4, Q9JIX3
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Bis(5'-adenosyl)-triphosphatase (FHIT) is a ~16 kDa protein. Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2.

1. Trapasso, F. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1592-7. PMID: 12574506
2. Huang, K. et al. (2004) Biochemistry 43, 7637-42. PMID: 15182206
3. Barnes, LD. et al. (1996) Biochemistry 35, 11529-35. PMID: 8794732
4. Lima, CD. et al. (1997) Science (New York, N.Y.) 278, 286-90. PMID: 9323207
5. Brenner, C. et al. (1997) Protein engineering 10, 1461-3. PMID: 9543008
6. Pace, HC. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5484-9. PMID: 9576908
9. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326
Примечание For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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