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Anti-FN3K Polyclonal Antibody (HV281014)

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Aperçu
Numéro de catalogueHV281014
Description
Anti-FN3K Polyclonal Antibody (HV281014) is a rabbit polyclonal antibody detecting FN3K in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human FN3K (Met1-Lys309).
Cible Protein-ribulosamine 3-kinase FN3K, Protein-psicosamine 3-kinase FN3K, FN3K, Fructosamine-3-kinase
Purification Purified by antigen affinity column.
Numéro d'accès Q9H479
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Fructosamine-3-kinase (FN3K) is a ~35 kDa protein. Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate. Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions. Involved in intracellular deglycation in erythrocytes. Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function. Also able to phosphorylate psicosamines and ribulosamines.

1. Delpierre, G. et al. (2000) Diabetes 49, 1627-34. PMID: 11016445
2. Szwergold, BS. et al. (2001) Diabetes 50, 2139-47. PMID: 11522682
3. Delpierre, G. et al. (2002) The Biochemical journal 365, 801-8. PMID: 11975663
4. Collard, F. et al. (2003) Diabetes 52, 2888-95. PMID: 14633848
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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