Anti-FUS Polyclonal Antibody (HW826014)

Name: Anti-FUS Polyclonal Antibody
Brand: abinScience
SKU: HW826014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Vista general

Número de catálogo

HW826014

Descripción

Anti-FUS Polyclonal Antibody (HW826014) is a rabbit polyclonal antibody detecting FUS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human FUS (Ser282-Ser367).

Objetivo

RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein, FUS, TLS

Purificación

Purified by antigen affinity column.

Número de acceso

P35637

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

RNA-binding protein FUS is a ~53 kDa protein. DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Also binds its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair.

1. Yamaguchi, A. et al. (2016) Scientific reports 6, 35195. PMID: 27731383

2. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132

3. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092

4. Zhou, Y. et al. (2013) PLoS genetics 9, e1003895. PMID: 24204307

5. Baechtold, H. et al. (1999) The Journal of biological chemistry 274, 34337-42. PMID: 10567410

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-FUS polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: HepG2 cell lysate