Anti-FUS Polyclonal Antibody (HW826014)

Name: Anti-FUS Polyclonal Antibody
Brand: abinScience
SKU: HW826014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HW826014

説明

Anti-FUS Polyclonal Antibody (HW826014) is a rabbit polyclonal antibody detecting FUS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human FUS (Ser282-Ser367).

ターゲット

RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein, FUS, TLS

精製

Purified by antigen affinity column.

アクセッション番号

P35637

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

RNA-binding protein FUS is a ~53 kDa protein. DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Also binds its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair.

1. Yamaguchi, A. et al. (2016) Scientific reports 6, 35195. PMID: 27731383

2. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132

3. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092

4. Zhou, Y. et al. (2013) PLoS genetics 9, e1003895. PMID: 24204307

5. Baechtold, H. et al. (1999) The Journal of biological chemistry 274, 34337-42. PMID: 10567410

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-FUS polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: HepG2 cell lysate