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Anti-GATA1 Polyclonal Antibody (HB017024)

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概要
カタログ番号HB017024
説明
Anti-GATA1 Polyclonal Antibody (HB017024) is a rabbit polyclonal antibody detecting Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; GATA1; ERYF1; GF1 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Frog, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant human GATA1 (Ala211-Asp300).
ターゲット Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; GATA1; ERYF1; GF1
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 P15976, P43429, P17679, P23767, P17678, P23768, P23769, Q924Y4, O09100, P23824
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Erythroid transcription factor (GATA1) is a ~42 kDa protein. Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.

1. Ludwig, LS. et al. (2022) Blood 139, 2534-2546. PMID: 35030251
2. Mizuta, S. et al. (2014) Genes to cells : devoted to molecular & cellular mechanisms 19, 28-51. PMID: 24245781
注意事項 For research use only
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参考文献
Formula
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