Anti-GBA Polyclonal Antibody (HY292014)

Name: Anti-GBA Polyclonal Antibody
Brand: abinScience
SKU: HY292014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY292014

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human GBA (Val117-Leu214).

Objetivo

SGTase, Cholesterol glucosyltransferase, GBA, Cholesteryl-beta-glucosidase, D-glucosyl-N-acylsphingosine glucohydrolase, GLUC, Imiglucerase, Beta-glucocerebrosidase, Lysosomal acid GCase, Beta-GC, Alglucerase, GC, Lysosomal acid glucosylceramidase, Acid beta-glucosidase

Purificación

Purified by antigen affinity column.

Número de acceso

P04062

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Lysosomal acid glucosylceramidase (GBA) is a ~59 kDa protein. Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (such as N-acylsphing-4-enine) and glucose. Plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation. Catalyzes the glucosylation of cholesterol, through a transglucosylation reaction where glucose is transferred from GlcCer to cholesterol. GlcCer containing mono-unsaturated fatty acids (such as beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred as glucose donors for cholesterol glucosylation when compared with GlcCer containing same chain length of saturated fatty acids (such as beta-D-glucosyl-N-octadecanoyl-sphing-4-enine).

1. Ron, I. et al. (2005) Blood cells, molecules & diseases 35, 57-65. PMID: 15916907

2. Akiyama, H. et al. (2013) Biochemical and biophysical research communications 441, 838-43. PMID: 24211208

3. Akiyama, H. et al. (2020) The Journal of biological chemistry 295, 5257-5277. PMID: 32144204

4. Bannink, S. et al. (2024) Journal of lipid research 65, 100670. PMID: 39395789

5. Vaccaro, AM. et al. (1997) The Journal of biological chemistry 272, 16862-7. PMID: 9201993

6. Magalhaes, J. et al. (2016) Human molecular genetics 25, 3432-3445. PMID: 27378698

7. Kitatani, K. et al. (2009) The Journal of biological chemistry 284, 12972-8. PMID: 19279011

8. Marques, AR. et al. (2016) Journal of lipid research 57, 451-63. PMID: 26724485

9. Boer, DE. et al. (2021) Journal of lipid research 62, 100018. PMID: 33361282

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-GBA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo