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Anti-GINS1 Polyclonal Antibody (HA800014)

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Aperçu
Numéro de catalogueHA800014
Description
Anti-GINS1 Polyclonal Antibody (HA800014) is a rabbit polyclonal antibody detecting DNA replication complex GINS protein PSF1; GINS complex subunit 1; GINS1; KIAA0186; PSF1 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human GINS1 (Met1-Ser196).
Cible DNA replication complex GINS protein PSF1; GINS complex subunit 1; GINS1; KIAA0186; PSF1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q14691, A4IFH4, Q9CZ15, Q7ZT47
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

DNA replication complex GINS protein PSF1 (GINS1) is a ~22 kDa protein. Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.

1. Kamada, K. et al. (2007) Nature structural & molecular biology 14, 388-96. PMID: 17417653
2. Cottineau, J. et al. (2017) The Journal of clinical investigation 127, 1991-2006. PMID: 28414293
3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425
4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004
5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328
6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-GINS1 Polyclonal Antibody

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