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Anti-GNAI2 Polyclonal Antibody (HB595014)

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Vista general
Número de catálogoHB595014
Descripción
Anti-GNAI2 Polyclonal Antibody (HB595014) is a rabbit polyclonal antibody detecting Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2; GNAI2B in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Dog, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human GNAI2 (Ala31-Phe355).
Objetivo Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2; GNAI2B
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P04899, P04897, P08752, P38400, P50147, P10824, P63096, P63097, B2RSH2, P50146, P27044, P08754
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Guanine nucleotide-binding protein G(i) subunit alpha-2 (GNAI2) is a ~40 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. Examples of interacting GPCRs include the adenosine A1 receptor/ADORA1, CNR1, and FPR2. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal.

1. Bai, CW. et al. (2024) Theranostics 14, 2190-2209. PMID: 38505600
2. Draper-Joyce, CJ. et al. (2018) Nature 558, 559-563. PMID: 29925945
3. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350
4. Zhu, Y. et al. (2022) Nature communications 13, 1775. PMID: 35365641
5. Komatsuzaki, K. et al. (2002) Biochemical and biophysical research communications 297, 898-905. PMID: 12359238
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-GNAI2 Polyclonal Antibody

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