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Anti-GNAI2 Polyclonal Antibody (HB595014)

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Aperçu
Numéro de catalogueHB595014
Description
Anti-GNAI2 Polyclonal Antibody (HB595014) is a rabbit polyclonal antibody detecting Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2; GNAI2B in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Dog, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human GNAI2 (Ala31-Phe355).
Cible Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2; GNAI2B
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P04899, P04897, P08752, P38400, P50147, P10824, P63096, P63097, B2RSH2, P50146, P27044, P08754
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Guanine nucleotide-binding protein G(i) subunit alpha-2 (GNAI2) is a ~40 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. Examples of interacting GPCRs include the adenosine A1 receptor/ADORA1, CNR1, and FPR2. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal.

1. Bai, CW. et al. (2024) Theranostics 14, 2190-2209. PMID: 38505600
2. Draper-Joyce, CJ. et al. (2018) Nature 558, 559-563. PMID: 29925945
3. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350
4. Zhu, Y. et al. (2022) Nature communications 13, 1775. PMID: 35365641
5. Komatsuzaki, K. et al. (2002) Biochemical and biophysical research communications 297, 898-905. PMID: 12359238
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-GNAI2 Polyclonal Antibody

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