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Anti-GNL2 Polyclonal Antibody (HD433014)

Anti-GNL2 Polyclonal Antibody
Anti-GNL2 Polyclonal Antibody
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Aperçu
Numéro de catalogueHD433014
Description
Anti-GNL2 Polyclonal Antibody (HD433014) is a rabbit polyclonal antibody detecting GNL2 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human GNL2 (Glu195-Arg455).
Cible Autoantigen NGP-1, GNL2, NGP1, Nucleolar GTP-binding protein 2
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q13823
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsQ13823
Contexte

Nucleolar GTP-binding protein 2 (GNL2) is a ~83 kDa protein. GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation. May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels.

1. Liang, X. et al. (2020) Nature communications 11, 3542. PMID: 32669547
2. Datta, D. et al. (2015) The Journal of biological chemistry 290, 21536-52. PMID: 26203195
Note For research use only
Images
  • Anti-GNL2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GNL2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: NIH3T3 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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