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Anti-GOT1 Polyclonal Antibody (HB695014)

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Anti-GOT1 Polyclonal Antibody
Anti-GOT1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHB695014
Description
Anti-GOT1 Polyclonal Antibody (HB695014) is a rabbit polyclonal antibody detecting GOT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Equus caballus, and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human GOT1 (Met1-Gln413).
Cible GOT1, Cysteine aminotransferase, cytoplasmic, Aspartate aminotransferase, cytoplasmic, Glutamate oxaloacetate transaminase 1, Cysteine transaminase, cytoplasmic, Transaminase A, cAspAT, cCAT
Purification Purified by antigen affinity column.
Numéro d'accès P17174
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Aspartate aminotransferase, cytoplasmic (GOT1) is a ~46 kDa protein. Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide.

1. Shen, H. et al. (2011) Journal of human genetics 56, 801-5. PMID: 21900944
2. Irino, Y. et al. (2016) Scientific reports 6, 36749. PMID: 27827456
Note For research use only.
Images
  • Anti-GOT1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GOT1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse heart lysate
    Lane 3: Mouse liver lysate
    Lane 4: Rat brain lysate
    Lane 5: Rat heart lysate
    Lane 6: Rat liver lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-GOT1 Polyclonal Antibody

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