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Anti-GPR4 Polyclonal Antibody (HA696014)

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Aperçu
Numéro de catalogueHA696014
Description
Anti-GPR4 Polyclonal Antibody (HA696014) is a rabbit polyclonal antibody detecting G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l; GPR4 in ELISA, IHC, WB. Suitable for Human, Bovine, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human GPR4 (Ile284-Gln362).
Cible G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l; GPR4
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P46093, Q1JQB3, P50132
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

G-protein coupled receptor 4 (GPR4) is a ~40 kDa protein. Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. Activated by an optimal pH of 6.8-7.2. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPR4 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. May also couple with G(i), G(q) and G(12)/G(13) G proteins.

1. Ludwig, MG. et al. (2003) Nature 425, 93-8. PMID: 12955148
2. Tobo, M. et al. (2007) Cellular signalling 19, 1745-53. PMID: 17462861
3. Rowe, JB. et al. (2021) The Journal of biological chemistry 296, 100167. PMID: 33478938
4. Howard, MK. et al. (2025) Cell 188, 671-687.e20. PMID: 39753132
5. Ma, Y. et al. (2025) Nature communications 16, 605. PMID: 39799123
6. Yue, X. et al. (2025) Cell research 35, 423-436. PMID: 40211064
7. Guo, L. et al. (2025) Molecular cell 85, 1658-1673.e7. PMID: 40215959
8. Chen, LN. et al. (2025) Molecular cell 85, 1640-1657.e8. PMID: 40215960
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-GPR4 Polyclonal Antibody

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