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Anti-GPR68 Polyclonal Antibody (HS932014)

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Vista general
Número de catálogoHS932014
Descripción
Anti-GPR68 Polyclonal Antibody (HS932014) is a rabbit polyclonal antibody detecting Ovarian cancer G-protein coupled receptor 1; OGR-1; G-protein coupled receptor 68; GPR12A; Sphingosylphosphorylcholine receptor; GPR68; OGR1 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human GPR68 (Leu285-Ala365).
Objetivo Ovarian cancer G-protein coupled receptor 1; OGR-1; G-protein coupled receptor 68; GPR12A; Sphingosylphosphorylcholine receptor; GPR68; OGR1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q15743, Q8BFQ3
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

G-protein coupled receptor 68 (GPR68) is a ~41 kDa protein. Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as phospholipase C. GPR68 is mainly coupled to G(q) G proteins and mediates production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Acts as a key mechanosensor of fluid shear stress and membrane stretch.

1. Ludwig, MG. et al. (2003) Nature 425, 93-8. PMID: 12955148
2. Xu, J. et al. (2018) Cell 173, 762-775.e16. PMID: 29677517
3. Huang, XP. et al. (2020) Biochemistry 59, 3594-3614. PMID: 32865988
4. Rowe, JB. et al. (2021) The Journal of biological chemistry 296, 100167. PMID: 33478938
5. Howard, MK. et al. (2025) Cell 188, 671-687.e20. PMID: 39753132
6. Guo, L. et al. (2025) Molecular cell 85, 1658-1673.e7. PMID: 40215959
7. Chen, LN. et al. (2025) Molecular cell 85, 1640-1657.e8. PMID: 40215960
Nota For research use only
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Working Solution
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Anti-GPR68 Polyclonal Antibody

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