Anti-GPX1 Polyclonal Antibody (HY365014)

Name: Anti-GPX1 Polyclonal Antibody
Brand: abinScience
SKU: HY365014
Price: 96 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY365014

Description

Anti-GPX1 Polyclonal Antibody (HY365014) is a rabbit polyclonal antibody detecting GPX1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Callithrix jacchus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human GPX1 (Thr50-Ala202).

Cible

Cellular glutathione peroxidase, Glutathione peroxidase 1, GPx-1, GPX1, GSHPx-1

Purification

Purified by antigen affinity column.

Numéro d'accès

P07203

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Glutathione peroxidase 1 (GPX1) is a ~22 kDa protein. Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.

1. Sutherland, M. et al. (2001) The Biochemical journal 353, 91-100. PMID: 11115402

2. Schwarz, M. et al. (2023) Redox biology 59, 102593. PMID: 36608588

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GPX1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Mouse liver lysate
Lane 3: Mouse kidney lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse lung lysate
Lane 6: Rat liver lysate
Lane 7: Rat kidney lysate

Reférences

Fiche technique

Anti-GPX1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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