Anti-GRSF1 Polyclonal Antibody (HB421014)

Name: Anti-GRSF1 Polyclonal Antibody
Brand: abinScience
SKU: HB421014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HB421014

Description

Anti-GRSF1 Polyclonal Antibody (HB421014) is a rabbit polyclonal antibody detecting G-rich sequence factor 1; GRSF-1; GRSF1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human GRSF1 (Val149-Ser335).

Cible

G-rich sequence factor 1; GRSF-1; GRSF1

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q12849, Q8C5Q4

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

G-rich sequence factor 1 (GRSF1) is a ~53 kDa protein. Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome.

1. Antonicka, H. et al. (2013) Cell metabolism 17, 386-98. PMID: 23473033

2. Jourdain, AA. et al. (2013) Cell metabolism 17, 399-410. PMID: 23473034

3. Pietras, Z. et al. (2018) Nature communications 9, 2558. PMID: 29967381

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-GRSF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate

Reférences

Fiche technique

Anti-GRSF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-GRSF1 Polyclonal Antibody (HB421014)

Aperçu

Images

Reférences

Fiche technique

SDS