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| カタログ番号 | HB421014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-GRSF1 Polyclonal Antibody (HB421014) is a rabbit polyclonal antibody detecting G-rich sequence factor 1; GRSF-1; GRSF1 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant human GRSF1 (Val149-Ser335). | ||||||||
| ターゲット | G-rich sequence factor 1; GRSF-1; GRSF1 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q12849, Q8C5Q4 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | G-rich sequence factor 1 (GRSF1) is a ~53 kDa protein. Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. 1. Antonicka, H. et al. (2013) Cell metabolism 17, 386-98. PMID: 23473033 2. Jourdain, AA. et al. (2013) Cell metabolism 17, 399-410. PMID: 23473034 3. Pietras, Z. et al. (2018) Nature communications 9, 2558. PMID: 29967381 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-GRSF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate
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