Anti-GSK3B Polyclonal Antibody (HW521014)

Name: Anti-GSK3B Polyclonal Antibody
Brand: abinScience
SKU: HW521014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW521014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human GSK3B (Met1-Thr420).

Objetivo

Glycogen synthase kinase-3 beta, GSK3B, Serine/threonine-protein kinase GSK3B, GSK-3 beta

Purificación

Purified by antigen affinity column.

Número de acceso

P49841

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Glycogen synthase kinase-3 beta (GSK3B) is a ~46 kDa protein. Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase.

1. Frame, S. et al. (2001) Molecular cell 7, 1321-7. PMID: 11430833

2. Dajani, R. et al. (2003) The EMBO journal 22, 494-501. PMID: 12554650

3. Cho, JH. et al. (2004) Journal of neurochemistry 88, 349-58. PMID: 14690523

4. Yin, L. et al. (2006) Science (New York, N.Y.) 311, 1002-5. PMID: 16484495

5. Boyle, WJ. et al. (1991) Cell 64, 573-84. PMID: 1846781

6. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854

7. Beals, CR. et al. (1997) Science (New York, N.Y.) 275, 1930-4. PMID: 9072970

8. Welsh, GI. et al. (1993) The Biochemical journal 294 ( Pt 3), 625-9. PMID: 8397507

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-GSK3B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo