Anti-GSK3B Polyclonal Antibody (HW521014)

Name: Anti-GSK3B Polyclonal Antibody
Brand: abinScience
SKU: HW521014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW521014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human GSK3B (Met1-Thr420).

Cible

Glycogen synthase kinase-3 beta, GSK3B, Serine/threonine-protein kinase GSK3B, GSK-3 beta

Purification

Purified by antigen affinity column.

Numéro d'accès

P49841

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Glycogen synthase kinase-3 beta (GSK3B) is a ~46 kDa protein. Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase.

1. Frame, S. et al. (2001) Molecular cell 7, 1321-7. PMID: 11430833

2. Dajani, R. et al. (2003) The EMBO journal 22, 494-501. PMID: 12554650

3. Cho, JH. et al. (2004) Journal of neurochemistry 88, 349-58. PMID: 14690523

4. Yin, L. et al. (2006) Science (New York, N.Y.) 311, 1002-5. PMID: 16484495

5. Boyle, WJ. et al. (1991) Cell 64, 573-84. PMID: 1846781

6. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854

7. Beals, CR. et al. (1997) Science (New York, N.Y.) 275, 1930-4. PMID: 9072970

8. Welsh, GI. et al. (1993) The Biochemical journal 294 ( Pt 3), 625-9. PMID: 8397507

Note

For research use only.

Images

Reférences

Fiche technique

Anti-GSK3B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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