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Anti-GTF2F1 Polyclonal Antibody (HA622014)

Anti-GTF2F1 Polyclonal Antibody
Anti-GTF2F1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHA622014
Description
Anti-GTF2F1 Polyclonal Antibody (HA622014) is a rabbit polyclonal antibody detecting General transcription factor IIF subunit 1; General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74; GTF2F1; RAP74 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human GTF2F1 (Met1-Asp190).
Cible General transcription factor IIF subunit 1; General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74; GTF2F1; RAP74
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P35269, Q5EA53, Q6AY96, Q3THK3
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

General transcription factor IIF subunit 1 (GTF2F1) is a ~58 kDa protein. TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Note For research use only
Images
  • Anti-GTF2F1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GTF2F1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Hela cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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