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Anti-GTF2F1 Polyclonal Antibody (HA622014)

Anti-GTF2F1 Polyclonal Antibody
Anti-GTF2F1 Polyclonal Antibody
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概要
カタログ番号HA622014
説明
Anti-GTF2F1 Polyclonal Antibody (HA622014) is a rabbit polyclonal antibody detecting General transcription factor IIF subunit 1; General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74; GTF2F1; RAP74 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant human GTF2F1 (Met1-Asp190).
ターゲット General transcription factor IIF subunit 1; General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74; GTF2F1; RAP74
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 P35269, Q5EA53, Q6AY96, Q3THK3
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

General transcription factor IIF subunit 1 (GTF2F1) is a ~58 kDa protein. TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

注意事項 For research use only
イメージ
  • Anti-GTF2F1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GTF2F1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Hela cell lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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