Anti-Human Granzyme A Polyclonal Antibody (HY375014)

Name: Anti-Human Granzyme A Polyclonal Antibody
Brand: abinScience
SKU: HY375014
Price: 168 USD
Availability: InStock

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Fiche technique
SDS

Aperçu

Numéro de catalogue

HY375014

Description

Anti-GZMA Polyclonal Antibody (HY375014) is a rabbit polyclonal antibody detecting GZMA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human GZMA (Ile29-Val262).

Cible

H factor, CTLA3, Cytotoxic T-lymphocyte proteinase 1, CTL tryptase, Fragmentin-1, HFSP, HF, Granzyme A, Granzyme-1, GZMA, Hanukkah factor

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Numéro d'accès

P12544

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Granzyme A (GZMA) is a ~28 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.

1. Hink-Schauer, C. et al. (2003) Nature structural biology 10, 535-40. PMID: 12819770

2. Zhou, Z. et al. (2020) Science (New York, N.Y.) 368. PMID: 32299851

3. Gershenfeld, HK. et al. (1988) Proceedings of the National Academy of Sciences of the United States of America 85, 1184-8. PMID: 3257574

4. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682

5. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427

6. Hansen, JM. et al. (2021) Cell 184, 3178-3191.e18. PMID: 34022140

7. Gong, W. et al. (2022) Genes & diseases 9, 1405-1407. PMID: 36157507

8. Oltra, SS. et al. (2023) Cell death and differentiation 30, 1366-1381. PMID: 36899106

Note

For research use only.

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