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Anti-H1-1 Polyclonal Antibody (HB348014)

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Vista general
Número de catálogoHB348014
Descripción
Anti-H1-1 Polyclonal Antibody (HB348014) is a rabbit polyclonal antibody detecting Histone H1.1; Histone H1a; H1-1; H1F1; HIST1H1A in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, Mouse, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human H1-1 (Ala39-Glu118).
Objetivo Histone H1.1; Histone H1a; H1-1; H1F1; HIST1H1A
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q02539, G3N131, D4A3K5, P43275, P16402, P43276, P09987, P16403, D3ZBN0, P43277, P10412, P15865, P43274
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Histone H1.1 (H1-1) is a ~21 kDa protein. Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex. Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

1. Lever, MA. et al. (2000) Nature 408, 873-6. PMID: 11130728
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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