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Anti-H6PD Polyclonal Antibody (HF993014)

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Aperçu
Numéro de catalogueHF993014
Description
Anti-H6PD Polyclonal Antibody (HF993014) is a rabbit polyclonal antibody detecting H6PD in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human H6PD (Gln20-Gln526).
Cible GDH, 6PGL, Glucose 1-dehydrogenase, H6PD, Glucose-6-phosphate dehydrogenase, GDH/6PGL endoplasmic bifunctional protein
Purification Purified by antigen affinity column.
Numéro d'accès O95479
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

GDH/6PGL endoplasmic bifunctional protein (H6PD) is a ~88 kDa protein. Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes. Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway. In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway. May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate. The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum.

1. Draper, N. et al. (2003) Nature genetics 34, 434-9. PMID: 12858176
2. Lavery, GG. et al. (2008) The Journal of clinical endocrinology and metabolism 93, 3827-32. PMID: 18628520
3. Lavery, GG. et al. (2013) European journal of endocrinology 168, K19-26. PMID: 23132696
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-H6PD Polyclonal Antibody

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