Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466074)

Name: Anti-HAdV-5 E1B-55K Polyclonal Antibody
Brand: abinScience
SKU: VK466074
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

VK466074

Descripción

Anti-HAdV-5 E1B-55K/E1B-495R Polyclonal Antibody (VK466074) is a rabbit polyclonal antibody detecting E1B-495R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5), Human adenovirus C serotype 2 (HAdV-2)

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant HAdV-5 E1B-55K/E1B-495R (Met1-Gln79).

Objetivo

E1B 55 kDa protein, E1B-55K, E1B protein, large T-antigen, E1B-495R

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

P03244, P03243

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

E1B 55 kDa protein (E1B-55K/E1B-495R) is a ~54 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest.

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-E1BB5-ADE05 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HAdV-5 E1B-55K/E1B-495R (Catalog No: VK466072)

Referencias

Datasheet

Anti-HAdV-5 E1B-55K Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo