Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466084)

Name: Anti-HAdV-5 E1B-55K Polyclonal Antibody
Brand: abinScience
SKU: VK466084
Price: 168 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK466084

Description

Anti-HAdV-5 E1B-55K/E1B-495R Polyclonal Antibody (VK466084) is a rabbit polyclonal antibody detecting E1B-495R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human adenovirus C serotype 2 (HAdV-2), Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant HAdV-5 E1B-55K/E1B-495R (Lys138-Val426).

Cible

E1B 55 kDa protein, E1B-55K, E1b55K, E1B protein, large T-antigen, E1B-495R

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P03243, P03244

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

E1B 55 kDa protein (E1B-55K/E1B-495R) is a ~55 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest.

1. Harada, JN. et al. (2002) Journal of virology 76, 9194-206. PMID: 12186903

2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032

3. Schwartz, RA. et al. (2008) Journal of virology 82, 9043-55. PMID: 18614635

4. Schreiner, S. et al. (2010) Journal of virology 84, 7029-38. PMID: 20484509

5. Pennella, MA. et al. (2010) Journal of virology 84, 12210-25. PMID: 20861261

6. Wimmer, P. et al. (2016) Oncogene 35, 69-82. PMID: 25772236

Note

For research use only

Images

Reférences

Fiche technique

Anti-HAdV-5 E1B-55K Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai