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| カタログ番号 | VK466034 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466034) is a rabbit polyclonal antibody detecting E1B-55K in ELISA, WB.
Highlights
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| 種反応性 | Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) | ||||||||
| アプリケーション | ELISA, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant HAdV-5 E1B-55K (Glu2-Gln79). | ||||||||
| ターゲット | E1B 55 kDa protein, E1B-55K, E1B protein, large T-antigen, E1B-495R | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P03243 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | E1B 55 kDa protein (E1B-55K) is a ~55 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest. 1. Harada, JN. et al. (2002) Journal of virology 76, 9194-206. PMID: 12186903 2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032 3. Schwartz, RA. et al. (2008) Journal of virology 82, 9043-55. PMID: 18614635 4. Schreiner, S. et al. (2010) Journal of virology 84, 7029-38. PMID: 20484509 5. Pennella, MA. et al. (2010) Journal of virology 84, 12210-25. PMID: 20861261 6. Wimmer, P. et al. (2016) Oncogene 35, 69-82. PMID: 25772236 | ||||||||
| 注意事項 | For research use only. |
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