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Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014)

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Vista general
Número de catálogoVK734014
Descripción
Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014) is a rabbit polyclonal antibody detecting Shutoff protein in ELISA, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Reactividad de especiesHuman adenovirus B serotype 7 (HAdV-7)
AplicacionesELISA, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant HAdV-7 Shutoff protein/L4 (Leu373-Arg537).
Objetivo Shutoff protein, 100 kDa protein, p100K, 100K-chaperone protein, L4-100K, Shutoff protein 100K, L4
Purificación Purified by antigen affinity column.
Número de acceso Q5EY60
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Shutoff protein (L4) is a ~92 kDa protein. Protein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers.

Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody

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