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Anti-HAS2 Polyclonal Antibody (HV192014)

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Aperçu
Numéro de catalogueHV192014
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human HAS2 (Glu67-Thr172).
Cible HA synthase 2, Hyaluronan synthase 2, Hyaluronic acid synthase 2, HAS2, Hyaluronate synthase 2
Purification Purified by antigen affinity column.
Numéro d'accès Q92819
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Hyaluronan synthase 2 (HAS2) is a ~63 kDa protein. Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan.

1. Karousou, E. et al. (2010) The Journal of biological chemistry 285, 23647-54. PMID: 20507985
2. Vigetti, D. et al. (2011) The Journal of biological chemistry 286, 7917-7924. PMID: 21228273
3. Rilla, K. et al. (2013) The Journal of biological chemistry 288, 5973-83. PMID: 23303191
4. Kasai, K. et al. (2020) Biochemical and biophysical research communications 533, 732-738. PMID: 32993960
5. Watanabe, K. et al. (1996) The Journal of biological chemistry 271, 22945-8. PMID: 8798477
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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