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Anti-HBV HBsAg/preS1 Recombinant Antibody (G10), FITC (VK539313)

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Vista general
Número de catálogoVK539313
Reactividad de especiesHepatitis B virus (HBV)
AplicacionesELISA, FCM
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1
ID de clonG10
ConjugaciónFITC
Objetivo Large envelope protein, L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen, S
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso Q9E6S4
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Large envelope protein (HBsAg) is a ~43 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane.

Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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