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Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5) (VK665043)

Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5)
Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5)
Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5)
Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5)
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Aperçu
Numéro de catalogueVK665043
Reactivité des espècesHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
ApplicationsELISA, WB
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneKE5
Cible Envelope glycoprotein B, gB, gB
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P13201, F5HB53, P06473
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Envelope glycoprotein B (gB) is a ~101 kDa protein. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.

Note For research use only
Images
  • Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5)

    Western blot

    Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HCMV/HHV5 Envelope glycoprotein B/gB(Catalog No: VK665011)
    Lane 2: negative control
    Lane 3: negative control

  • Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5)

    Bioactivity

    Detects recombinant HCMV gB protein (Catalog No: VK665011) in indirect ELISA

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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