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Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014)

Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody
Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoVK457014
Descripción
Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Bovine coronavirus and Equine coronavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman coronavirus OC43 (HCoV-OC43)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant HCoV-OC43 NP/Nucleoprotein (Met1-Ile448).
Objetivo N, NC, Nucleocapsid protein, Nucleoprotein, Protein N
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P33469
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Nucleoprotein (NP) is a ~49 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
Nota For research use only
Imágenes
  • Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NCAP-CVHOC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HCoV-OC43 NP/Nucleoprotein (Catalog No: VK457012)

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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