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Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014)

Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody
Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody
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  • 50ug
  • 100ug
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Aperçu
Numéro de catalogueVK457014
Description
Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Bovine coronavirus and Equine coronavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman coronavirus OC43 (HCoV-OC43)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant HCoV-OC43 NP/Nucleoprotein (Met1-Ile448).
Cible N, NC, Nucleocapsid protein, Nucleoprotein, Protein N
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P33469
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Nucleoprotein (NP) is a ~49 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
Note For research use only
Images
  • Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NCAP-CVHOC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HCoV-OC43 NP/Nucleoprotein (Catalog No: VK457012)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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