Anti-HDAC3 Polyclonal Antibody (HT281014)

Name: Anti-HDAC3 Polyclonal Antibody
Brand: abinScience
SKU: HT281014
Price: 168 USD
Availability: InStock

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100ug

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Aperçu

Numéro de catalogue

HT281014

Description

Anti-HDAC3 Polyclonal Antibody (HT281014) is a rabbit polyclonal antibody detecting HDAC3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Danio rerio, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human HDAC3 (Met1-Gln376).

Cible

HD3, SMAP45, RPD3-2, Histone deacetylase 3, HDAC3

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

O15379

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Histone deacetylase 3 (HDAC3) is a ~48 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

1. Chini, CC. et al. (2010) The Journal of biological chemistry 285, 40830-7. PMID: 21030595

2. Pillai, VB. et al. (2011) Molecular and cellular biology 31, 2349-63. PMID: 21444723

3. Hatzi, K. et al. (2013) Cell reports 4, 578-88. PMID: 23911289

4. Yi, J. et al. (2014) The Journal of biological chemistry 289, 33878-86. PMID: 25301942

5. Lee, DY. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 2072-2077. PMID: 28167758

6. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810

7. Zhan, W. et al. (2020) Oncogenesis 9, 48. PMID: 32404892

8. Watson, PJ. et al. (2012) Nature 481, 335-40. PMID: 22230954

10. Yuan, ZL. et al. (2005) Science (New York, N.Y.) 307, 269-73. PMID: 15653507

Note

For research use only.

Images

Reférences

Fiche technique

Anti-HDAC3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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