Anti-HDAC3 Polyclonal Antibody (HT281014)

Name: Anti-HDAC3 Polyclonal Antibody
Brand: abinScience
SKU: HT281014
Price: 168 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

スペック:

50ug
100ug

数量:

お問い合わせ

概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HT281014

説明

Anti-HDAC3 Polyclonal Antibody (HT281014) is a rabbit polyclonal antibody detecting HDAC3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Danio rerio, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human HDAC3 (Met1-Gln376).

ターゲット

HD3, SMAP45, RPD3-2, Histone deacetylase 3, HDAC3

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

O15379

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Histone deacetylase 3 (HDAC3) is a ~48 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

1. Chini, CC. et al. (2010) The Journal of biological chemistry 285, 40830-7. PMID: 21030595

2. Pillai, VB. et al. (2011) Molecular and cellular biology 31, 2349-63. PMID: 21444723

3. Hatzi, K. et al. (2013) Cell reports 4, 578-88. PMID: 23911289

4. Yi, J. et al. (2014) The Journal of biological chemistry 289, 33878-86. PMID: 25301942

5. Lee, DY. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 2072-2077. PMID: 28167758

6. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810

7. Zhan, W. et al. (2020) Oncogenesis 9, 48. PMID: 32404892

8. Watson, PJ. et al. (2012) Nature 481, 335-40. PMID: 22230954

10. Yuan, ZL. et al. (2005) Science (New York, N.Y.) 307, 269-73. PMID: 15653507

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-HDAC3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-HDAC3 Polyclonal Antibody (HT281014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ