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Anti-HDAC8 Polyclonal Antibody (HV385014)

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Vista general
Número de catálogoHV385014
Descripción
Anti-HDAC8 Polyclonal Antibody (HV385014) is a rabbit polyclonal antibody detecting HDAC8 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HDAC8 (Met1-Val377).
Objetivo HDAC8, Histone deacetylase 8, HD8, HDACL1
Purificación Purified by antigen affinity column.
Número de acceso Q9BY41
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Histone deacetylase 8 (HDAC8) is a ~41 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.

1. Hu, E. et al. (2000) The Journal of biological chemistry 275, 15254-64. PMID: 10748112
2. Van den Wyngaert, I. et al. (2000) FEBS letters 478, 77-83. PMID: 10922473
3. Buggy, JJ. et al. (2000) The Biochemical journal 350 Pt 1, 199-205. PMID: 10926844
4. Lee, H. et al. (2004) Molecular and cellular biology 24, 765-73. PMID: 14701748
5. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HDAC8 Polyclonal Antibody

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