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Anti-HEV Met-Y Methyltransferase Recombinant Antibody (SAA2696) (VK620083)

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Aperçu
Numéro de catalogueVK620083
Reactivité des espècesHepatitis E virus (HEV)
ApplicationsELISA
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1
ID de cloneSAA2696
Cible EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.4.-.-, EC:3.6.4.-, Methyltransferase, Non-structural polyprotein pORF1
Niveau d'endotoxines <10 EU/mg
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès Q9YLR1
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Non-structural polyprotein pORF1 (MeT-y Methyltransferase) is a ~187 kDa protein. Methyltransferase: Displays a capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m(7)GTP or m(7)GDP. GDP is a better substrate than GTP.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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