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Anti-HIC1 Polyclonal Antibody (HA798014)

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Vista general
Número de catálogoHA798014
Descripción
Anti-HIC1 Polyclonal Antibody (HA798014) is a rabbit polyclonal antibody detecting Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29 in ELISA, IHC, WB. Suitable for Human, Mouse, and Zebrafish.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human HIC1 (Lys561-Thr733).
Objetivo Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q14526, Q9R1Y5, Q9JLZ6, Q90W33, Q96JB3
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Hypermethylated in cancer 1 protein (HIC1) is a ~76 kDa protein. Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses.

1. Deltour, S. et al. (2002) Molecular and cellular biology 22, 4890-901. PMID: 12052894
2. Pinte, S. et al. (2004) The Journal of biological chemistry 279, 38313-24. PMID: 15231840
3. Zhang, W. et al. (2010) Oncogene 29, 2467-76. PMID: 20154726
4. Chen, WY. et al. (2005) Cell 123, 437-48. PMID: 16269335
5. Van Rechem, C. et al. (2010) Molecular and cellular biology 30, 4045-59. PMID: 20547755
6. Valenta, T. et al. (2006) The EMBO journal 25, 2326-37. PMID: 16724116
7. Briggs, KJ. et al. (2008) Genes & development 22, 770-85. PMID: 18347096
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HIC1 Polyclonal Antibody

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