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Обзор
Каталожный номерHA798014
Описание
Anti-HIC1 Polyclonal Antibody (HA798014) is a rabbit polyclonal antibody detecting Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29 in ELISA, IHC, WB. Suitable for Human, Mouse, and Zebrafish.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant human HIC1 (Lys561-Thr733).
Цель Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q14526, Q9R1Y5, Q9JLZ6, Q90W33, Q96JB3
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Hypermethylated in cancer 1 protein (HIC1) is a ~76 kDa protein. Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses.

1. Deltour, S. et al. (2002) Molecular and cellular biology 22, 4890-901. PMID: 12052894
2. Pinte, S. et al. (2004) The Journal of biological chemistry 279, 38313-24. PMID: 15231840
3. Zhang, W. et al. (2010) Oncogene 29, 2467-76. PMID: 20154726
4. Chen, WY. et al. (2005) Cell 123, 437-48. PMID: 16269335
5. Van Rechem, C. et al. (2010) Molecular and cellular biology 30, 4045-59. PMID: 20547755
6. Valenta, T. et al. (2006) The EMBO journal 25, 2326-37. PMID: 16724116
7. Briggs, KJ. et al. (2008) Genes & development 22, 770-85. PMID: 18347096
Примечание For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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