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| カタログ番号 | HS839014 | ||||||||
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| 説明 |
Anti-HIST2H2BE/H2BC21 Polyclonal Antibody (HS839014) is a rabbit polyclonal antibody detecting HIST2H2BE in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anopheles gambiae, and Asterias rubens.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human HIST2H2BE/H2BC21 (Pro2-Lys126). | ||||||||
| ターゲット | H2B/q, Histone H2B-GL105, H2B-clustered histone 21, Histone H2B.q, H2BC21, Histone H2B type 2-E, HIST2H2BE, H2BFQ | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q16778 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Histone H2B type 2-E (HIST2H2BE/H2BC21) is a ~13 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | ||||||||
| 注意事項 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting HIST2H2BE (HS839014) at a concentration of 13.2 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of HIST2H2BE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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