Anti-Histone H3 Polyclonal Antibody (HV603014)

Name: Anti-Histone H3 Polyclonal Antibody
Brand: abinScience
SKU: HV603014
Price: 96 USD
Availability: InStock

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Reférences
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SDS

Aperçu

Numéro de catalogue

HV603014

Description

Anti-Histone H3 Polyclonal Antibody (HV603014) is a rabbit polyclonal antibody detecting Histone H3/o in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Aspergillus niger.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human H3C15 (Met1-Ala136).

Cible

HIST2H3A, H3C15, Histone H3/o, H3-clustered histone 14, H3-clustered histone 15, H3-clustered histone 13, H3F2, H3FM, Histone H3/m, HIST2H3D, HIST2H3C, Histone H3.2

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q71DI3

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Histone H3.2 (H3C15) is a ~15 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-H3C15 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Mouse spleen lysate

Reférences

Fiche technique

Anti-Histone H3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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