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| カタログ番号 | HV603014 | ||||||
|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Histone H3 Polyclonal Antibody (HV603014) is a rabbit polyclonal antibody detecting Histone H3/o in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Aspergillus niger.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||
| アプリケーション | ELISA, IHC, WB | ||||||
| 宿主種 | Rabbit | ||||||
| クローン性 | Polyclonal | ||||||
| アイソタイプ | IgG | ||||||
| 免疫原 | E. coli - derived recombinant Human H3C15 (Met1-Ala136). | ||||||
| ターゲット | HIST2H3A, H3C15, Histone H3/o, H3-clustered histone 14, H3-clustered histone 15, H3-clustered histone 13, H3F2, H3FM, Histone H3/m, HIST2H3D, HIST2H3C, Histone H3.2 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 精製 | Purified by antigen affinity column. | ||||||
| アクセッション番号 | Q71DI3 | ||||||
| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 背景 | Histone H3.2 (H3C15) is a ~15 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | ||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-H3C15 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Mouse spleen lysate
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