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Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422) (VK497013)

Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422)
Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422)
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Aperçu
Numéro de catalogueVK497013
Description
Anti-HIV-1 rev/ART/TRS protein Nanobody (SAA1422) [SAA1422] (VK497013) is a alpaca monoclonal antibody detecting ART/TRS in ELISA, IF.
Reactivité des espècesHuman immunodeficiency virus type 1 (HIV-1)
ApplicationsELISA, IF
Espèce hôteAlpaca
ClonalitéMonoclonal
IsotypeVHH-8His-Cys-tag
ID de cloneSAA1422
Cible Protein Rev, ART/TRS, Anti-repression transactivator, Regulator of expression of viral proteins, rev
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Numéro d'accès P04618
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein Rev (ART/TRS) is a ~13 kDa protein. Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1.

Note For research use only.
Images
  • Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422)

    SDS-PAGE

    SDS-PAGE for Anti-HIV-1 rev/ART/TRS protein Nanobody (SAA1422)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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