中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB947014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-HK1 Polyclonal Antibody (HB947014) is a rabbit polyclonal antibody detecting HK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
|
||||||||
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human HK1 (Phe80-Met215). | ||||||||
| ターゲット | Hexokinase type I, Hexokinase-1, HK1, Brain form hexokinase, Hexokinase-A, HK I | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P19367 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Hexokinase-1 (HK1) is a ~102 kDa protein. Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome. 1. Magnani, M. et al. (1992) The Biochemical journal 285 ( Pt 1), 193-9. PMID: 1637300 2. Wang, F. et al. (2014) Investigative ophthalmology & visual science 55, 7159-64. PMID: 25316723 3. Wolf, AJ. et al. (2016) Cell 166, 624-636. PMID: 27374331 | ||||||||
| 注意事項 | For research use only. |
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
